RAPD-PCR Analysis of Streptomyces sp. with Novel Antibacterial Activity

ISSN: 0973-7510

E-ISSN: 2581-690X

RAPD-PCR Analysis of Streptomyces sp. with Novel Antibacterial Activity

Mohammed A. Ibrahim¹ , Sahar Abdul-Wahab², Rima Albesharat², Haithum W. Al-Obaide³, Mohannad M. Al-Obaidi⁴ and Khulud Alsouleman²

¹Royal Scientific Society, Environmental Research Center, Al-Jubeiha 11941 Jordan.

²National Commission of Biotechnology, Damascus, Syria.

³Carnegie Institute, Troy, Michigan, USA.

⁴College of Medicine, Baghdad University, Baghdad, Iraq.

J Pure Appl Microbiol. 2009;3(2):411-414

Received: 23/05/2009 | Accepted: 15/07/2009| Published: 31/10/2009

Abstract

Random amplified polymorphic DNA (RAPD) analysis was applied to ten bioactive and non bioactive Streptomyces isolates (RW series) recovered from Syrian soils. The Streptomyces isolates were characterized with different patterns of antibacterial activities against Gram positive and negative bacteria. Nine of 22 RAPD decamer primers which were used in the study were found to amplify Streptomyces genomic DNA, and revealed interesting DNA polymorphism in the Streptomyces genomes of the isolates. RAPD- PCR products generated twenty six DNA fragments with 290 to 1081 base pairs. RAPD-PCR analysis of Streptomyces sp. RW6/2 genomic DNA showed that the PCR products of primer OPZ-19 gave one specific fragment of 521 base pairs which was not detected in other Streptomyces isolates.

Keywords

Antibacterial activity, DNA polymorphism, RAPD-PCR, Streptomyces, Vibrio fluvialis

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