ISSN: 0973-7510

E-ISSN: 2581-690X

C.M. Ginoya and N.M. Gohel
Department of Plant Pathology, B. A. College of Agriculture, Anand Agricultural University, Anand – 388 110, India.
J. Pure Appl. Microbiol., 2016, 10 (1): 183-190
© The Author(s). 2016
Received: 09/11/2015 | Accepted: 06/01/2016 | Published: 31/03/2016
Abstract

Genetic diversity in chilli fruit rot pathogen (Alternaria alternata) was analyzed using eight isolates collected from major chilli growing regions of Gujarat. The genomic DNA extracted from each isolates of Alternaria alternata was subjected to polymerase chain reaction using 65 random decamer primers from OPA, OPD and OPF series. Only 10 of the 65 RAPD primers were selected based on repeatability. The 10 earmarked RAPD primers selected from these series amplified 214 DNA fragments with size ranging from 119.93 to 3236.45 bp. Out of these, 98 were polymorphic giving 98.98 per cent polymorphism. The total number of amplified fragments varied from 19 in OPA-3 to 25 in OPA-13. The average polymorphic bands per primer were 9.8 and percent polymorphism ranged from 85.71 in OPF-1 to 100 in rest of the primers. The PIC value varied from 0.746 in OPF-1 to 0.910 in OPA-16. Dendrogram generated by pooled molecular data of 10 RAPD primers formed two clusters namely ‘A’ and ‘B’. The cluster ‘A’ included Aa-1 and Aa-2 isolates, while the cluster ‘B’ included Aa-3, Aa-4, Aa-5, Aa-6, Aa-7 and Aa-8 isolates. Thus, the molecular characterization of eight isolates of A. alternata by RAPD revealed existence of variations.

Keywords

Random Amplification of Polymorphic DNA, Molecular diversity, Alternaria alternata, Chilli fruit rot.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.