ISSN: 0973-7510

E-ISSN: 2581-690X

Fohad Mabood Husain1,2, Iqbal Ahmad2 , Nasser Abdulatif Al-Shabib1, Riyazuddin1, Mohd Shahnawaz Khan3, Yahya Ahmed Mohammed4 and Abdullah Safar Althubiani5
1Department of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh-11541, Kingdom of Saudi Arabia.
2Department of Agricultural Microbiology, Aligarh Muslim University, Aligarh – 202 002, India.
3Department of Biochemistry, College of Science, King Saud University,
Riyadh-11541, Kingdom of Saudi Arabia.
4College of Engineering, King Saud University, Riyadh-11541, Kingdom of Saudi Arabia.
5Department of Biology, Faculty of Applied Science, Umm Al-Qura University, Makkah-21955, Kingdom of Saudi Arabia.
J. Pure Appl. Microbiol., 2016, 10 (3): 1745-1754
© The Author(s). 2016
Received: 13/01/2016 | Accepted: 19/04/2016 | Published: 30/09/2016
Abstract

Indiscriminate use of antibiotics has led to development of drug resistance among infection causing bacteria rendering the current antibiotic therapy ineffective. This has compelled the scientific community across the globe to look for new drug targets. Since quorum sensing plays a key in the expression of virulence genes and biofilm formation it can be a potential target for curing infections caused by pathogens. Therefore, keeping in mind the ubiquitous an diverse nature of quorum quenching bacteria, date palm rhizospheric soil of Saudi Arabia was selected to screen for quorum quenching bacteria. In ther present study, SBF1 strain demonstrated promising inhibition of violacein Chromobacterium violaceum 12472 biosensor strain and was selected for anti-virulence assay. Using 16S rDNA analysis the strain SBF1 was identified as Bacillus amyloliquefaciens (GenBank Accession Number: KC494392). Culture extract of SBF1 demonstrated dose-dependent (0.75–6 mg/ml) reduction against the QS regulated violacein production in C. violaceum (45–79%) and LasB elastase (4.9-63.8%), total protease (34.3–84.4%) and pyocyanin (32.7-63.7%) in PAO1. The extract also decreased the swarming migration of CV12472 and PAO1 significantly. Biofilm forming ability of PAO1 was also significantly impaired after treatment with SBF1 extract. Ring opening assay suggests that the anti-QS activity is due the production of lactonase enzyme. Findings of the present study demonstrate the anti-QS and antibiofilm potential of SBF 1 extract. Quorum quenching ability of SBF1 can be exploited to treat bacterial infections, to prevent food spoilage and in bioremediation.

Keywords

Bacillus amyloliquefaciens, biofilm, date palm, lactonase, quorum sensing, virulence.

Article Metrics

Article View: 291
PDF Download: 79

Share This Article

Journal Tools

© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.