In view to show a simple DNA purification step from agarose gel, we applied the DNA bandstab technique to the 16S-23S Intergenic Spacer Region (ISR) (a polymorphic region resulting in multiple bands after gel-electrophoresis) of selected Lactobacillus spp. The short ISR was successfully extracted by bandstab after numerous unsuccessful attempts of DNA cutting-elution techniques. The purification of band was further confirmed by the distinctive peaks observed in the DNA chromatograms generated after the sequencing procedure.
Bandstab-PCR, Lactobacillus, 16S-23S intergenic spacer region, Sequencing
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