ISSN: 0973-7510

E-ISSN: 2581-690X

Ying Tang, Di Huang, Chang Hai Wang
and Li Ming Ma

1School of Life Science and Biotechnology, Dalian University of technology, Linggong Road 2, Dalian, Liaoning, 116024, China.
J. Pure Appl. Microbiol. 2014, 8(6):4351-4363
© The Author(s). 2014
Received: 06/04/2014 | Accepted: 19/08/2014 | Published: 31/12/2014
Abstract

Investigations of microalgae-bacteria relationship are important to studies of microbial ecology, red tide, aquaculture and pollutant degradation, etc. To date, microalgae-bacteria interactions are still not well understood, and purification of eukaryotic microalgae is also rarely mentioned. Here, bacteria-free Dunaliella salina was obtained with optimized antibiotic treatments. Axenic microalgal growth did not differ significantly with non-axenic algae in f/2 medium. However, when the contents of nitrogen and phosphorus were 10-fold higher of f/2, or there were no vitamins or trace elements in f/2 medium, the non-axenic algae grow much faster-increased 6.78%, 10.25% and 10.19% than axenic algal biomass, respectively. Associated bacteria haven’t significant effect on microalgal intracellular gross fat and ash contents, while improved microalgal intracellular protein and intra- and extra-cellular total carbohydrate contents during most growth stages. Associated bacteria didn’t impact algal nitrogen uptake. While under high nutrient concentration conditions, the phosphorus contents decreased more rapidly in non-axenic algal culture (decreased 40.48%) than in axenic algal culture (decreased 5.99%) within the first 4 days, suggesting that non-axenic algal phosphorus uptake was stronger. The study provided a simpler system and effective references to research of microalgae-bacteria relationship.

Keywords

Antibiotics, Bacteria-microalga interaction; Biochemistry composition, Dunaliella salina,  Inorganic nutrients uptake, Purification

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© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.