ISSN: 0973-7510
E-ISSN: 2581-690X
In the present study the lapinized swine fever vaccine virus was propagated in the PK-15 cell line. Indirect fluorescent antibody test, indirect immunoperoxidase test and sandwich ELISA were employed for demonstration of the virus in cell culture. The comparative efficacy of the three tests was also evaluated. The cell culture adapted virus was concentrated and purified using polyethylene glycol (PEG) treatment and sucrose gradient centrifugation. A total of seven fractions could be collected out of which four fractions were found to be viable. The purified viable virus having 10 5.25 TCID 50 per ml was successfully used for raising Swine Fever virus specific antibodies in rabbits and pigs.
ELISA, Lapinized SFV, PK-15, PEG, Sucrose gradient
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