ISSN: 0973-7510

E-ISSN: 2581-690X

Sujata Chavan and M.M.V. Baig
1Department of Botany and Department of Biotechnology, Yeshwant Mahavidyalaya, Nanded – 431 602, India.
J Pure Appl Microbiol. 2014;8(2):1095-1099
© The Author(s). 2014
Received: 29/11/2013 | Accepted: 21/01/2014 | Published: 31/04/2014
Abstract

Thirty physiologically variant pathogenic strains of Xanthomonas sp from various orchards in and around Nanded district, Maharashtra were isolated. Of the thirty strains, one strain isolated from Citrus lemona from Nanded district, Maharashtra was found to be the most effective in the pectinolytic enzymes production. This strain was subjected for production of pectinolytic enzymes in surface culture and various factors affecting the enzyme production was studied. The optimum time for production of pectinolytic enzymes by this strain was 48 hrs. The optimum temperature and pH for production of pectinolytic enzymes by this strain was 30°c and 7 respectively. The effect of various carbon and nitrogen sources was studied and it was found that pectin was most suitable carbon source and yeast extract was most suitable nitrogen source for optimum production of pectinolytic enzymes.

Keywords

Xanthomonas sps, pectinolytic enzymes

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