ISSN: 0973-7510

E-ISSN: 2581-690X

Dakshayani Lomada1 , Suneetha Nuthakki2, J. Venkata Narayana2 and G. Venkateswerlu3
1Department of Genetics & Genomics, Yogi Vemana University, Kadapa – 516003, India.
2Joginpally B R Engineering College, Yenkapally, Ranga Reddy (District), Andhra Pradesh, India.
3Department of Biochemistry, Osmania University, Hyderabad – 500 007, India.
J Pure Appl Microbiol. 2014;8(1):497-504
© The Author(s). 2014
Received: 21/03/2013 | Accepted: 26/05/2013 | Published: 28/02/2014

Chitinases have received substantial attention owing to their potential application in environmental protection for clearing the waste generated in shrimp and crab processing industry. We report a chitinase producing fungus isolated from soil samples of Andhra Pradesh, India, where shrimp shells were dumped regularly. The isolated fungus was identified as a Malbranchea chrysosporioidea and an extracellular chitinase was purified from the culture filtrate of Malbranchea chrysosporioidea. The purified chitinase has a molecular weight of 100 kDa as estimated by SDS-PAGE further being confirmed by activity staining using glycol chitin in the gel. The isoelectric point of purified enzyme was 8.45. The enzyme exhibited an optimum pH of 9.0 and a temperature of 370C, with swollen chitin as the substrate. The enzyme was unusually activated by Cd2+ and inhibited by Zn2+, Co2+ and As3+. Results of RP-HPLC analysis of hydrolytic products revealed a unique nature of purified chitinase, exhibiting exochitinase and b-N-acetyl hexosaminidase activities releasing chitobiose and N-acetylglucosamine respectively as end products. To our knowledge, this is the first report on purification and characterization of a chitinase from Malbranchea chrysosporioidea isolated from soil of Andhra Pradesh, India.


Malbranchea chrysosporioidea, Chitinase purification, Exochitinase and β-N-acetyl hexosaminidase activities

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© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.