ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access

Muslu Selman1, Genc Berna2, Adiguzel Mahmut3, Albayrak Seyda1 and Adiguzel Ahmet1

1Department of Molecular Biology and Genetics, Faculty of Science, Ataturk University, 25240, Erzurum, Turkey.
2Department of Genetic and Bioengineering, Faculty of Engineering and Life Sciences, Gumushane University, 29100, Gumushane, Turkey.
3Department of Environmental Engineering, Faculty of Engineering, Ataturk University, 25400, Erzurum, Turkey.
J. Pure Appl. Microbiol., 2020, 14 (1): 63-72 | Article Number: 5991
https://doi.org/10.22207/JPAM.14.1.09 | © The Author(s). 2020
Received: 11/12/2019 | Accepted: 07/01/2020 | Published: 20/01/2020
Abstract

Enzymes which would be active in cold conditions can be used in a wide range of fields from molecular biology to detergent industry due to their low processing capacity and high activity. In this study, sixty cold-adapted bacteria were isolated from water and sludge samples collected from Erzurum and Van provinces. Identification of eight isolates by molecular [(GTG)5-PCR and 16S rRNA sequencing] techniques and tests for temperature (4-35°C), pH (3-11) and salt (2-15% (w/v) requirements were performed. These bacteria were belonging to Pseudomonas chlororaphis subsp. aureofaciens (SM011A), Psychrobacter faecalis (SM012D), Rahnella aquatilis (SM015A), Shewanella putrefaciens (SM018A), Pseudomonas lactis (SM0110A), Flavobacterium chryseum (SM0112E), Exiguobacterium mexicanum (SM0117A) and Glutamicibacter arilaitensis (SM0118A). The physicochemical requirements for all isolates ranged between 4-25°C, pH 5-7 and 2-15% salt (NaCl) concentration. However, E. mexicanum did not require salt in growth medium. All bacteria were evaluated for protease, lipase and amylase enzymes and all were found to be multiple enzyme producers. The eight isolates were identified from the resources of Turkey, for the first time and enzyme production abilities of some isolates to produce enzymes were declared. The originating of the producers of these enzymes from Turkey shows that Turkey has a remarkable reservoir for cold-adaptive microorganisms and these microorganisms will make important contributions to the detergent industry worldwide.

Keywords

Cold-adaptive, Industrial potential, Molecular characterization, Multiple enzyme.

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