ISSN: 0973-7510

E-ISSN: 2581-690X

Lirong Yan1, Ming Yu1, Wei Han1, Yanhua Chen1, Hongtu Zhou1, Wenbing Wang2 and Mandi Miao1
1Affiliated Hospital of Jiangsu University, Zhenjiang – 212 001, Jiangsu Province, China.
2Institute of life science, Jiangsu University – 212 013, China.
J Pure Appl Microbiol. 2014;8(1):635-639
© The Author(s). 2014
Received: 08/04/2013 | Accepted: 25/05/2013 | Published: 28/02/2014
Abstract

To determine the protective effects of ethanol extract from termite fungus garden on the SH-SY5Y cell against oxidative stress induced by H2O2. SH-SY5Y cells were cultured in the medium as the normal control group. H2O2 was added into SH-SY5Y cells to establish the cell model of oxidative damage as the model control group. Different concentrations of ethanol extract from termite fungus garden and the reagent H2O2 were added into the culture medium of SH-SY5Y in sequence as the prevention groups. Ethanol extract from termite fungus garden added into the cell culture medium induced by H2O2 was considered as the protection groups. Cell activity of SH-SY5Y was determined with MTT assay, and the activities of SOD (Superoxide Dismutase), GSH (Glutathione), and content of MDA (Malondialdehyde)  were also detected in each group. Cell suruvival rate in the prevention group had been increased by 27.2% to 44.5% as compared with that in the model control group. All of the protection groups had 24.2% to 32.5% improvement on survival rate as compared with that in the model control group. The ethanol extract from termite fungus garden plays an important role in protecting SH-SY5Y cell through its anti-oxidative effect on H2O2.

Keywords

termite fungus garden, antioxidant, SH-SY5Y

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