Ten strains were isolated from first rain soil deposits in and around Nagavali River basin and screened for protease production. One strain producing maximum protease was selected and identified as Lysinibacillus sp. by 16S rRNA gene sequencing. In order to enhance the production of protease, the effects of major medium ingredients carbon and nitrogen sources on the production of the enzyme were investigated. Amongst all carbon sources used, fructose showed the highest potential for the production. The best organic nitrogen source observed was skim milk. Inorganic nitrogen sources were not as effective as organic sources. Increased production (51%) of the enyzme was obtained by manipulating the medium composition. The optimum pH and temperature for the purified enzyme activity was 7.0 and 55°C respectively. The study of its stability showed that the enzyme is stable in the alkaline pH range i.e. 6.0-9.0. A single band with a molecular weight of 25 kDa was resolved employing SDS-PAGE analysis.
Protease, 16S rRNA, river basin, Lysinibacillus
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