ISSN: 0973-7510
E-ISSN: 2581-690X
Ten strains were isolated from first rain soil deposits in and around Nagavali River basin and screened for protease production. One strain producing maximum protease was selected and identified as Lysinibacillus sp. by 16S rRNA gene sequencing. In order to enhance the production of protease, the effects of major medium ingredients carbon and nitrogen sources on the production of the enzyme were investigated. Amongst all carbon sources used, fructose showed the highest potential for the production. The best organic nitrogen source observed was skim milk. Inorganic nitrogen sources were not as effective as organic sources. Increased production (51%) of the enyzme was obtained by manipulating the medium composition. The optimum pH and temperature for the purified enzyme activity was 7.0 and 55°C respectively. The study of its stability showed that the enzyme is stable in the alkaline pH range i.e. 6.0-9.0. A single band with a molecular weight of 25 kDa was resolved employing SDS-PAGE analysis.
Protease, 16S rRNA, river basin, Lysinibacillus
© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.