Polycyclic aromatic hydrocarbons are accumulated in the environment due to a variety of anthropogenic activities. In this study, 330 bacterial strains isolated from rhizosphere of Calotropis sp., which were growing in industrial, farming and natural sites, were investigated for their ability to grow in the presence of polycyclic aromatic hydrocarbons added to minimal salt basal medium. Four isolates were found to be capable of using naphthalene, anthracene, chrysene, benzene, toluene and xylene as sole source of carbon. The taxonomic position of these isolates was examined by amplification and sequencing of 16S rDNA gene. The isolates were found to be belonging to Bacillus sp. The mean growth rate constant (K) of isolates were found to increase with successive increase in substrate concentration (0.5 to 1.0 mg/50ml). KS37 (B. cereus PPB6) degraded 81.39% of anthracene while, KS141 (Bacillus sp.WJ21) and KS146 (Bacillus sp. MC-BAC2) degraded 65.30 %, 69.71 % of naphthalene respectively after 6 days of incubation as determined by HPLC analysis. The results obtained indicate that the rhizosphere of Calotropis is a rich source of Bacillus sp., which have potential to degrade the PAH molecules.