Propagation of a Field Isolate of Avian Pox Virus on Chorioallantoic Membrane of Developing Chicken Embryo and in BGM-70 Cell Line

ISSN: 0973-7510

E-ISSN: 2581-690X

R.K. Verma¹ , R.K. Joshi², A.K. Gangwar³, Namita Joshi⁴ and S. Shukla⁵

¹Department of Veterinary Teaching Veterinary Clinical Complex (T.V.C.C), College of Veterinary Science and Animal Husbandry N.D. University of Agriculture and Technology Kumarganj- 224229, Faizabad, India

²Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry N.D. University of agriculture and Technology Kumarganj- 224229, Faizabad, India

³Department of Veterinary Surgery and Radiology, College of Veterinary Science and
Animal Husbandry N.D. University of agriculture and Technology Kumarganj- 224229, Faizabad, India

⁴Department of Veterinary Public Health , College of Veterinary Science and Animal
Husbandry, Narendra Deva University of Agriculture and Technology, Kumarganj, Faizabad – 224229, India.

⁵Department of Biosciences, Integral University, Lucknow (U.P.) 226026, India.

J Pure Appl Microbiol. 2015;9(1):169-174

Received: 06/04/2014| Accepted: 19/08/2014| Published: 31/03/2015

Abstract

A field isolate of Fowl pox virus (FPV) was grown successfully on chorioallantoic membrane (CAM) of developing chicken embryo and Baby Gravid Monkey Kidney cell line (BGM-70 cells), as evident by different degree of Cytopathic effects (CPE). Studies were conducted on the pathogenicity of these viruses in these different host systems which produced pock lesions in CAM infected with FPV with extensive congestion and foci of hemorrhages covering entire inoculated site. The cytopathic effects in infected BGM-70 cell monolayers consisted of rounding and clumping of cells, vacuolation, macro and micro plaques and intracytoplasmic inclusion bodies. The cytopathic effects were seen in both unstained as well as MGG stained monolayers of BGM-70 cells

Keywords

Fowlpox virus (FPV), Chorioallantoic membrane (CAM) Pock lesions, Cytopathic effects (CPE), BGM-70

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