Production of poly-(3-hydroxybutyrate) by Bacillus sp. 112A isolated from activated sludge sample using flours of ten different pulses and cereals, such as, pigeon pea, red lentil, black gram, bengal gram, green gram, corn, soya bean, wheat, rice and sorghum available in abundant as spills was in a range of 1.20 g/L to 0.14 g/L. Bacillus sp. 112A was able to accumulate a maximum of 1.20 g/L PHB within 30 h of incubation when corn flour (25 g/L) was used as carbon substrate suggesting a very faster rate of polymer synthesis when compared to all other substrates.