Bacterial cellulose (BC) or nata is produced by the Gram negative bacterium Acetobacter xylinum at the air liquid interface of sugary rich liquid medium. In the present investigation, BC was produced from Hestrin Schramn (HS) medium and Modified Coconut Liquid Endosperm (MCLE) medium using the efficient cellulose producing isolate of sugarcane juice (sju-1) under static batch fermentation condition. The standard culture Acetobacter xylinum NCIM was also compared for the production of BC. Results showed that sju-1 produced BC of 14.10 and 15.40 g/l in HS medium and MCLE medium respectively after 14 days of fermentation period. NCIM 2526 produced only 11.10 and 9.80 g/ l of cellulose. The biosynthetic yield of cellulose was more in MCLE medium when compared to the HS medium and proved to be highly significant. BC obtained was highly juicy and rich in crude fibre. Water holding capacity of BC ranged from 84.44 % to 87.80% and the crude fibre content in the range 10.74 to 11.25 g. The results clearly confirmed that the coconut liquid endosperm medium could effectively be used as potential alternative to the glucose normally used in the media of BC production and the Acetobacter xylinum sju-1 isolate produced bacterial cellulose in quantities of commercial interest.