The production of tannase by Aspergillus niger GH1 in solid-state fermentation, under different initial concentrations of tannic acid (12.5, 25, 50 and 100 gl-1). The reactors were packed with polyurethane foam impregnated with medium and inoculated with fungal spores. Tannase production was kinetically monitored by 48 h. The tannase activities extracellular and intracellular were assayed by HPLC-chromatography. Maximum extracellular and intracellular tannase activities (11.35 and 6.95 Uml-1 respectively) were recorded with 100 gl-1 of tannic acid. The substrate uptake was 100% at concentrations of 12.5, 25 and 50 gl-1, while 74.4% was consumed in the presence of 100 gl-1 of tannic acid after 48 h of culture. These results suggest that high concentrations of tannins can be removed and tannase production can be reached in high levels. The needed to establish the production conditions under solid state fermentation, a system where the tannase is expressed extracellular in high levels.
Tannase, Tannic acid, Solid-state fermentation, Aspergillus niger GH1
© The Author(s) 2009. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.