ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Sawsan Abd Ellatif1, Eman Abdullah M. Ali2 , Hoda H. Senousy2 and Elsayed S. Abdel Razik3
1Bioprocess Development Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City for Scientific Research and Technology Application, New Borg El-Arab, 21934, Alexandria, Egypt.
2Botany and Microbiology Department, Faculty of Science, Cairo University, 12613, Giza, Egypt.
3Plant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City for Scientific Research and Technology Application, New Borg El-Arab,21934,Alexandria, Egypt.
J. Pure Appl. Microbiol., 2019, 13 (4): 1985-1994 | Article Number: 5849
https://doi.org/10.22207/JPAM.13.4.10 | © The Author(s). 2019
Received: 15/09/2019 | Accepted: 29/10/2019 | Published: 10/12/2019
Abstract

Arbuscular mycorrhizal fungi form obligate symbiotic associations with most of plant families. This limits their in vitro culturing and large-scale production. In vitro root organ culture is very useful for studying these symbiotic relationships and for mass production of arbuscular mycorrhizal fungal inoculants. This research aimed to study the effect of different phenolic compounds on the growth of the arbuscular mycorrhizal fungus Gigaspora gigantea using in vitro tomato root organ culture. Eight phenolic compounds were used against control without phenolic compounds. The phenolic compounds used in this research were cinnamic acid, catechin anhydrous, protocatechuic acid, ferulic acid, tannic acid, coumarin, esculetin and catechol. The experiments were done at two different pHs (5.7 and 6.5) in both solid and liquid media. Phenolic compounds exhibited different effects including stimulatory, inhibitory or no effects. Catechin anhydrous (which is a flavonoid compound) showed the most significant increase in both mycorrhizal root colonization and arbuscular abundance with moderate growth of root hairs at pH 6.5. Tannic acid inhibited the growth of root hairs, mycorrhizal colonization and formation of arbuscules at pH 5.7. Solid media were superior to liquid media in both mycorrhizal colonization and arbuscular formation.

Keywords

Arbuscular mycorrhizal fungi, Gigaspora gigantea, Phenolic compounds, In vitro root organ culture, Symbiosis, Transformation.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.