Production and partial purification of alpha amylase by mesophilic mold Acremonium sporosulcatum. Using substrate starch solution pH 4.5 with 0.2 M sodium acetate buffer. The production maximum in maize starch, it has optimum growth temperature at 30 ^oC, the pH 7.0 was found to be the optimum for the best synthesis of the enzyme. The influence of incubation temperature was studied by growing different temperature 20^o, 30 ^o, 35 ^o, and 37 ^oC. Fermentation carried out using potato, sweet potato, maize, maize cob powder, rice, and tapioca starch in different concentrations of sugar present in the basal medium. 7.5% of starch is for maximum yield, for nitrogen source among inorganic sodium acetate and organic yeast extract is found best production. In effect of aeration 50 ml is carried in 250 ml flask, partial purification of enzyme was active in wide range of values in pH 5-8 optimum activity exhibited at pH 7.0 and temperature 70 ^oC after the enzyme inactive slowly in 15 minutes for incubation at 100 ^oC it completely loss activity