ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Fatin Syahirah Othman1 , Haryati Jamaluddin2 , Zaharah Ibrahim2 , Hirofumi Hara1 , Nurul Ashyikin Yahya1 , Koji Iwamoto1 and Shaza Eva Mohamad3
1Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, 54100, Jalan Sultan Yahya Petra, Kuala Lumpur, Malaysia.
2Faculty of Science, Universiti Teknologi Malaysia, 81310, UTM Johor Bahru, Johor, Malaysia.
3International Centre, Tokyo City University, 3-3-1 Ushikubo Nishi Tsuzuki-ku, Yokohama, Kanagawa, 224-8851 Japan.
J Pure Appl Microbiol, 2019, 13 (3): 1297-1306 | Article Number: 5773 | © The Author(s). 2019
Received: 09/08/2019 | Accepted: 17/09/2019 | Published: 29/09/2019

The main purpose of this study is to provide information on the biochemical composition of the total lipid extract of three different native freshwater microalgae namely Acutodesmus obliquus CN01, Chlorella sp. Carolina-15-2069, and Chlorella vulgaris NIES-1269 at different growth phases, cultured under standardized conditions that will be useful for commercialization purposes due to the content of a-linolenic acid (ALA), a type of omega-3 fatty acids. We monitored growth, biomass accumulation, chlorophyll content, lipid content and fatty acids composition in all three freshwater microalgae. In addition, this study seeks to throw some light on the lipid content of a newly isolated freshwater green alga Acutodesmus obliquus CN01 which have shown to give significant quantities of ALA, an added nutritional value to this microalga oil. Acutodesmus obliquus CN01 exhibited the fastest growth rate among the three microalgae used. Biomass was shown to be enhanced in the nitrogen enrichment medium. The outcomes from this study indicated that newly isolated Acutodesmus obliquus CN01 from Malaysia cultured under normal condition showed fast growth rate with high lipid content and also high ALA content. Therefore, from this study, Acutodesmus obliquus CN01 was identified as species of choice to be further explored for its potential to produce omega-3 fatty acids.


Acutodesmus obliquus, Chlorella sp., Chlorella vulgaris, Lipid, Malaysia.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.