A Streptomyces strain isolated from Beni-Suef wastewater treatment plant reported previously as a potent producer of proteases was subjected to fermentation for production of the protease, fractional precipitation of the enzyme by ammonium sulphate, purification of the enzyme by sephadex G200 and characterization of the enzyme by SDS-PAGE gel electrophoresis. The results showed that the purified protease enzyme produced by the strain Streptomyces C11 gave only a single band on the SDS-PAGE gel with a molecular weight of about 50 kDa reflecting its purity and homogeneity. The results showed that the wastewater actinomycete strain Streptomyces C11 have good capabilities for the production of a pure and homogenous protease.