ISSN: 0973-7510

E-ISSN: 2581-690X

Khushbu Patel1 , Hiren Patel2 and Gaurav Shah1
1Department of Biotechnology, Veer Narmad South Gujarat University, Surat-395 007, India.
2Department of Plant Molecular Biology and Biotechnology, Navsari Agricultural University, Navsari-396 450, India.
J Pure Appl Microbiol. 2014;8(5):4163-4169
© The Author(s). 2014
Received: 21/04/2014 | Accepted: 07/06/2014 | Published: 31/10/2014
Abstract

The main purpose of this study to reduced the production cost of cellulase by using alternative carbon source such as agricultural waste residue and optimized fermentation medium for high yielding. The present investigation was carried out to isolate the novel cellulase producing fungi, CPF-1 & CPF-2 with optimization of physicochemical and nutritional parameters for cellulase production. Among agricultural residues, rice bran and wheat straw were found to be best carbon source for the production of cellulase by CPF-1 and CPF-2, respectively.  The optimal parameter (Temp, pH and incubation time) for the production of cellulase by CPF-1 and CPF-2 were 280C, pH 6 & 120 min and 280C, pH 7 & 96 min respectively. Also the thermal stability of both the purified cellulase enzymes was high at 600C for 85min & 70 min for CPF-1 and CPF-2 fungi respectively.

Keywords

Agricultural waste, Biodegradation, Cellulase, Fermentation, Optimization

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