ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Ashraf S.A. El-Sayed1 , Manal T. El Sayed1, Hend S. Nada2, Abdallah E. Hassan3 and Eman K. Yousef3
1Microbiology Department, Faculty of Science, Zagazig University, 44519, Egypt.
2Microbiology and Immunology Department, Faculty of Veterinary Medicine, Zagazig University, 44519, Egypt.
3Chemistry Department, Faculty of Science, Zagazig University, 44519, Egypt.
J. Pure Appl. Microbiol., 2019, 13 (4): 2055-2063 | Article Number: 5855
https://doi.org/10.22207/JPAM.13.4.17 | © The Author(s). 2019
Received: 18/09/2019 | Accepted: 19/10/2019 | Published: 16/12/2019
Abstract

Taxol, a diterpenoid was initially isolated from the bark of Taxus brevifolia, approved by FDA in 1994 as a powerful drug for metastatic ovarian carcinoma, breast and lung cancer. However, due to limitations in the production of this drug based on this plant source, the productive potentiality of fungi of this compound opened a new avenue for its commercial production. In this study, among the twenty fungal isolates screened for Taxol production, Aspergillus terreus had the highest potentiality to produce Taxol (131.2 µg/ml). The productivity of Taxol by A. terreus has been maximized by nutritional optimization using inhibitors and growth regulators. The yield of Taxol by A. terreus was maximally obtained (0.663µg/ml) by growing the fungal isolate on potato dextrose broth medium, amended with addition of biotin at 150 µg/ml for 20 days. The chemical structure Taxol extracted of A. terreus has been verified by proton and carbon NMR, IR and UV analyses. The activity of A. terreus Taxol has been assessed towards multiple cell lines, displaying a strong anticancer activity. In conclusion, the productivity of Taxol by A. terreus has been greatly improved upon using biotin as chemical modulator, that open a new avenue for commercializing the Taxol yield by fungi.

Keywords

Aspergillus terreus; Taxol; Nutritional optimization, Inhibitors.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.