Production and Characterisation of Cellulase by Streptobacillus species- APS-8

ISSN: 0973-7510

E-ISSN: 2581-690X

Production and Characterisation of Cellulase by Streptobacillus species- APS-8

S.V. Rajagopal¹ , K. Siva Kumar, A. Uma Sankar², M. Rama Rao³ and S. Sunitha Kumari

¹Department of Biotechnology, College of Science, GITAM University, Visakhapatnam – 530 045, India.

²Department of Microbiology, College of Science, GITAM University, Visakhapatnam – 530 045, India.

³Department of Biochemistry, College of Science, GITAM University, Visakhapatnam – 530 045, India.

J Pure Appl Microbiol. 2007;1(2):231-236

Received: 12/09/2007 | Accepted: 17/10/2007 | Published: 30/10/2007

Abstract

A cellulolytic bacterial species APS-8 was isolated from the soils of Visakhapatnam region. This strain is characterized as Gram positive Streptobacillus species. The strain grew well in medium containing Carboxymethylcellulose (CMC), producing maximum enzyme at 37°C and pH 8.0. The activity of extra cellular enzyme was found to be high at temperature 50°C and at pH 5.0. Metal ions like MgSO₄, ZnSO₄ and FeCl₃ enhanced the enzyme activity whereas MnSO₄ inhibited the enzyme activity under same assay conditions.

Keywords

Cellulase, Congo red, Carboxymethylcellulose (CMC), Carboxymethylcellulase (CMCase) assay, APS-8

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