ISSN: 0973-7510

E-ISSN: 2581-690X

R. Chandra and M.R. Rajan
Department of Biology, Gandhigram Rural University, Gandhigram – 624 302, India.
J Pure Appl Microbiol. 2009;3(1):363-366
© The Author(s). 2009
Received: 10/01/2009 | Accepted: 20/02/2009| Published: 30/04/2009

Two distinct colonies were isolated from the intestine of Koi carp Cyprinus carpio var. koi through serial dilution and pour plate method. Based on biochemical tests the isolated colonies were identified as Lacto bacillus and Proteus spp. Among the identified microbes mass multiplication of Lacto bacillus in nutrient broth was carried out. Five different feeds having different concentrations of Lacto bacillus such as Feed I ( control), Feed II( 1 ml), Feed III ( 2ml ), Feed IV ( 3ml ) and Feed V (4ml) were prepared by using fish meal, groundnut oil cake, wheat flour and tapioca flour. Role of microbes on feed utilization parameters were studied for a period of 30 days. The growths, percentage growth, relative growth rate, gross and net growth efficiency were higher in feed II containing 1 ml of microbes. Feed consumption, assimilation and metabolism were higher in control. Feed conversion efficiency was higher in feed III containing 2ml of Lacto bacillus. Feed conversion ratio was higher in feed IV.


Probiotic effect, intestinal bacteria, koi carp

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