ISSN: 0973-7510

E-ISSN: 2581-690X

Divya Sharma1, Ajay Kumar1 and Amit Kumar2
1Department of Microbiology, Shoolini Institute of Life Sciences and Business Management, Solan, Himachal Pradesh – 173212, India.
2Faculty of Biotechnology, Shoolini University of Biotechnology and Management Sciences,
Solan, Himachal Pradesh – 173212, India.
J Pure Appl Microbiol. 2014;8(3):2473-2484
© The Author(s). 2014
Received: 12/08/2013 | Accepted: 30/10/2013 | Published: 31/06/2014
Abstract

Fermented foods and beverages are consumed from very early time and health benefits are associated with their use. The traditional fermented foods and beverages are rich repository of potential microbial diversity. Keeping in view this, in the present study nineteen native isolates (14 bacterial and 5 yeast) obtained from various traditional fermented foods and beverages of Himachal Pradesh. Except for isolate 2IIPcbII all the 13 bacterial isolates produced bacteriocin and showed antibacterial activity against tested pathogenic bacteria. All the isolates were found to be tolerant to simulated gastric and intestinal juices as well as tolerant to ox-bile. The most efficient probiotic bacterial isolate 2IIIPcbI showed 1.12 and 0.04 viability difference in the presence of gastric and intestinal juices, respectively. This isolate also showed high survivability (0.511) in the presence of Ox bile (0.3%) after 3 hrs of incubation. Out of 14 bacterial isolates only four isolates viz., 2IPcbI, 2IIIPcbI, 4PcbII and IIBcbI showed bile salt deconjugation activity (glycocholic acid and taurocholic acid). Only 11 isolates showed adhesion to hydrocarbon and isolate 2IIIPcbI showed 92.45% adhesion activity. All the tested bacterial isolates showed auto-aggregation property and most potential isolate 2IIIPcbI showed 90.36% activity. On the basis of 16S rDNA sequencing the isolate 2IIIPcbI was identified as Bacillus subtilis. Out of 5 isolated yeast strains, the isolate IBcyII showed highest alcohol production (10%) as compared to other yeast strains. This most efficient alcohol producer IBcyII was identified as Saccharomyces cerevisiae on the basis of sequencing of ITS region.

Keywords

Alcohol production, Bacillus subtilis, Fermented foods & beverages,
ITS region, Probiotic, Saccharomyces cerevisiae, 16S rDNA.

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