ISSN: 0973-7510

E-ISSN: 2581-690X

B. Kiran Gandhi , R.H. Patil and Y. Srujana
Department of Entomology, University of Agricultural Sciences, Dharwad – 580 005, India.
J. Pure Appl. Microbiol., 2016, 10 (3): 2333-2340
© The Author(s). 2016
Received: 07/02/2016 | Accepted: 14/04/2016 | Published: 30/09/2016

As part of a metagenomic analysis of gut microflora, V3 region of the 16s rRNA gene of the bihar hairy caterpillar, Spilosoma obliqua collected from the sunflower field was sequenced using a MiSeq desktop sequencer (Illumina, San Diego, California). The sequence comprised of 381 074 paired-end reads with 50.76 % GC content and 35.95 average base quality (Phred score), after the reads were passed through a conserved region filter, a spacer sequence filter, a read quality filter, and mismatch filter. Singleton and chimeric sequences were removed as part of pre-processing, and the 255 904 paired-end reads that remained were considered for the analysis. After removing singleton operational taxonomic units (OTUs) and chimeric sequences, 206 OTUs were identified, representing 13 phyla, 22 classes, 32 orders, 48 families, 38 genera, and 16 species. To our knowledge, this is the first successful application of Illumina-based de novo sequencing for the analysing the microbial community of the gut of a bihar hairy caterpillar.


Gut, Metagenomic, Microflora, Operational taxonomic units.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.