ISSN: 0973-7510

E-ISSN: 2581-690X

Farhad Safarpoor Dehkordi , Yousef Valizadeh, Tannaz Alipour Birgani and Khashayar Ghasemi Dehkordi
1Young Researchers and Elites Club, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran.
J Pure Appl Microbiol. 2014;8(2):1065-1069
© The Author(s). 2014
Received: 12/07/2013 | Accepted: 29/08/2013 | Published: 31/04/2014
Abstract

Ingestion of raw infected milk and dairy products is the most important way for human brucellosis. This study was carried out in order to investigate the prevalence of Brucella spp., Brucella abortus and Brucella melitensis in cow’s milk using culture, dot-ELISA and duplex PCR.  All of the 150 milk samples were cultured for Brucella spp. and the positive results have been evaluated for presences of Brucella abortus and Brucella melitensis using dot-ELISA and duplex PCR assays. Totally, 12% of milk samples were positive for Brucella spp. using culture. The dot-Elisa assay indicated that 12% and 2% of milk samples were positive for Brucella abortus and Brucella melitensis, respectively. Novel duplex PCR showed that 14%, 4% and 2% of milk samples were positive for Brucella abortus, Brucella melitensis and both bacteria. Isfahan had the highest incidence of Brucella spp. (17.5%), while Chaharmahal Va Bakhtiari had the lowest incidence of Brucella spp. (8%). There were significant differences about P < 0.05 for incidence of Brucella spp. between Isfahan with Chaharmahal Va Bakhtiari provinces. Our study showed that milk inspection should be performed in order to diminish the distribution of brucellosis in human populations.

Keywords

Brucellosis, Milk samples, Culture, Dot-ELISA, Duplex PCR

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© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.