Research Article | Open Access
T. Jayaprakash1 , K.V. Leela1, A.Sundaram2, C. Panchapakesa Rajendran3 and R. Aparna1
1Department of Microbiology, SRM Medical College Hospital and Research Centre. Kattankulathur, Chennai – 603 203, Tamilnadu, India.
2SRM Medical College Hospital and Research Centre. Kattankulathur, Chennai – 603 203. Tamilnadu, India.
3Department of Rheumatology, SRM Medical College Hospital and Research Centre. Kattankulathur, Chennai – 603 203. Tamilnadu, India.
J Pure Appl Microbiol. 2021;15(1):382-389 | Article Number: 6568 | © The Author(s). 2021
Received: 04/08/2020 | Accepted: 19/02/2020 | Published: 23/02/2021

The present study aims to determine the spectrum of etiological agents and to screen HLA-B27 and, related inflammatory markers in patients presenting with symptoms of Spondyloarthritis(SpA) post diarrhea, which can aid in prompt diagnosis of reactive arthritis (ReA). A total of 903 stool samples of patients presenting with diarrhea were collected and screened with microscopic and culture techniques to identify the etiological agents. Blood samples of patients presenting with both diarrhea and arthritis were collected and subjected to C- reactive protein(CRP), Erythrocyte sedimentation rate(ESR) and Human Leukocyte Antigen B27(HLA-B27) detection tests.Among the total of 903 patients, 20 Salmonella species were isolated. Othergut microbes identified included Escherichia coli 572(63%), Klebsiella species 126(14%), Proteus vulgaris 43(5%), Proteus mirabilis 27(3%), Citrobacter species and Enterococcus species 8(1%), while some of these organisms(3.2%) caused inflammation resulting in ReA. Parasitic etiology was found in 99 patients, among which the most common parasites include Entamoeba histolytica and hookworm, identified through microscopy. Among the total, 29 patients were found to have symptoms of joint pain with articular and extra articular manifestations, and some associated with HLA-B27.


Reactive arthritis, HLA-B27, Salmonella spp., Gut microbes, Diarrhea


Reactive arthritis(ReA), defined as sterile synovitis, develops after a distant infection and may or may not be HLA-B27 associated. The detection of microbial components in the joints of patients with ReA, has led to the redefinition of ReA as an immune-mediated synovitis. It can result from slow bacterial infections with the presence of immunologic bacterial antigens and/or viable non-culturable bacteria in the intra-articular spaces, produced by metabolically active bacteria residing in the body, usually in the gastrointestinal or urogenital tract [Mustafa Serdar SA1,Colmegna I.2]. The clinical manifestations may include articular sites characterized by asymmetrical inflammation in the axial and peripheral joints and extra-articular manifestations such as urethritis, eye infections, skin rashes, or mucocutaneous manifestations [Schiellerup P.3, Connor BA.4].

Infections caused by Salmonella, Shigella, or Yersinia have been significantly associated with the risk of ReA, while itis uncommon in infections caused by Campylobacter or E. coli [Schiellerup P.3, Ekman P.5]. Other factors such as age, sex, bacterial load, as well as the presence or absence of HLA B27, may also influence the susceptibility for ReA [Schiellerup P.3, Ekman P.5].

Recent studies on ReA have focused on the post-infection complications caused by enteric pathogens such as Escherichia coli,Salmonella species,Campylobacter jejuni, Clostridium difficile, Entamoeba histolytica, Giardia lamblia, and Cryptosporidium [Buret AG.6, Keating RM.7, Boice JL.8, Woo P.9, Brocq O.10, Meadow11, Garcia-Kutzbach12] Following an enteric microbial infection, ReA has been reported in 1% of patients [Rohekar S.13] to 62% [Fendler C.14].

Periodic infections or delivery of bacterial antigen(s)to the joints may directly induce the activation of immune system which may result in the production of antigenic epitopes with cross-reactivity. Delivery of bacterial antigens, known as molecular mimicry, may induce the activation of cell-mediated immunity, in particular, cytotoxic T lymphocytes (CTLs), which show reactivity to HLA-B27 and also comprises of protein sequences analogous to those detected in bacteria associated in arthritis. Antibodies with reactivity to HLA-B27 have been detected in patients with arthritis [Harrison15, Hannu T.16, Ajene AN.17].

A high degree of genetic polymorphism has been observed in HLA-B27[Khan MA.18]. The diagnostic markers of enteric pathogens are related to the basic inflammatory markers correlated with the predisposing genetic marker of HLA-B27. It helps to identify the geographical distribution of genetic sub-type variations, where the patients are referred to a tertiary care centre.

Materials and Methods

All the patients with diarrhea, who were admitted to Sri Ramaswamy Memorial Medical College Hospital and Research Centre (SRM MCH & RC), Kattankulathur, Tamilnadu, between January 2018 and December 2019, were recruited in the study. All patients ≥18 years were made to understand the purpose of the test, assured of the confidentiality of the results, before obtaining the signed consent. Patients who did not have symptoms of spondyloarthritis post-diarrhea; and patients who weren’t available for follow up, were excluded from the study. All patients with diarrhea and symptoms of spondyloarthritides were diagnosed by the rheumatologist (Dr. C Panchapakesa Rajendran) and sent to the laboratory for further investigations. Stool specimens of all the 1108 patients were collected and transported in glucose phosphate broth and processed using culture methods as per CLSI guidelines, by the microbiologist (1st author, Jayaprakash T.).

Within 2 h of collection, the samples were processed by laboratory technicians. Stool samples were macroscopically observed for the presence of blood and/or mucus, followedby Gram staining using the modified Hucker’s method to easily detect the morphology of any bacteria present [Humphries RM.19]. Direct wet-mount preparation was carried out with the stool samples, by adding 2-3 drops of 0.85% saline and examined under a 22-22mm coverslip to detect motile trophozoites. The iodine-mount method was also used to provide a better contrast to detect the presence of bile-stained eggs. Fecal concentration methods were also used, as mentioned in Garcia LS et al. 2018; the sedimentation technique was performed and observed as wet preparation under the microscope using 10x and 40x objectives, with and without the presence of iodine [Garcia L.S.20].

As routine specimen processing, stool samples were inoculated in Selenite F broth, to enrich the salmonella species present. Samples were plated on MacConkey (MAC) media – to differentiate between lactose fermenting and non-lactose fermenting enteropathogens. Selective media such as Xylose Lysine Deoxycholate agar (XLD), and Deoxycholate Citrate agar (DCA) were used – to detect and differentiate the recovery of Salmonella, Shigella species, 5% Sheep blood agar (SBA) was used – to detect the presence of Vibrio spp., Plesiomonas spp., and Aeromonas spp., [Sanchez-Romero MI (21), S.A.22].

Phenotypic characteristics of the colonies were noted down and compared their consistency with that of enteric pathogens. Furthermore, the selective colonies were screened using biochemical tests such as, Indole test –to aid in differentiate various Enterobacteriaceae members and between P. mirabilis and P. vulgaris, Mannitol Motility Medium (MMM) – to determine the ability of the bacteria to ferment mannitol, and detect motility of the organisms, Triple Sugar Iron (TSI) test – to detect the production of hydrogen sulfide, gas, and the ability to ferment glucose, lactose and/or sucrose; Christenson’s urea agar test – to distinguish Proteus spp. from other enteric bacteria; and Simmons’ citrate medium test; to determine the ability of the organisms to use citrate as its sole carbon source, thus differentiating members of Enterobacteriaceae. All the isolates identified were further subjected to antimicrobial susceptibility tests to detect any resistance to the battery of antimicrobials used. All the culture techniques were performed with the quality control strains obtained from American Type Culture Collection (ATCC).

Among the total(1108), 205 patients were excluded as they were not available for follow-up, while the remaining 903 patients with diarrhea were followed up for 3-4 weeks, to check whether they had articular complaints of axial(unilateral or bilateral sacroiliitis, neck pain) and peripheral joints(shoulder, elbow, wrist, finger, thigh, knee, foot, heel and dorsum of foot pain), followed by extra-articular symptoms such as uveitis, and psoriasis. Laboratory evaluation including Human Leukocyte antigen (HLA-B27), C-reactive protein (CRP) and Erythrocyte Sedimentation Rate (ESR) were done in 29 patients who fulfilled the classification criteria for spondyloarthritis by Assessment of Spondyloarthritis International Society(ASAS). There are no universally accepted classification criteria specific for reactive arthritis and may vary with studies.

Blood samples of patients suspected with reactive arthritis29 were collected by a phlebotomist under aseptic conditions, 2ml in ethylenediamine tetraacetic acid(EDTA) tubes and 2ml in plain blood vacutainers. The EDTA samples were tested for ESR and for the detection of HLA-B27, while the blood collected in plain vacutainers were used to test for CRP using latex agglutination methods.

HLA-B27 (genetic marker)detection was performed by a microbiologist (Jayaprakash T.) using a Chip based Real-Time PCR (RT-PCR) method, based on the principle of TaqMan assay. DNA extraction from whole blood samples collected from patients in EDTA tubes was performed with Trueprep® AUTO/AUTO v2 Universal Cartridge based Sample Prep Device and Trueprep® AUTO/AUTO v2 Universal Cartridge based Sample Prep kit, which uses a proprietary matrix enclosed in a cartridge to purify nucleic acid from clinical samples.

The Truenat™ HLA-B27 chip labelled with patient ID is placed on the chip tray, away from the analyzer, without touching the reaction well. The cap of the microtube containing freeze-dried PCR reagents is removed, and using the filter barrier tip, 6µL of purified DNA is transferred into the microtube. The tube was allowed to stand for 30-60s to get a clear solution. The clear solution (6 µL) was dispensed into the reaction well of Truenat™ HLA-B27 chip, and the test was initiated.  At the end of the test run, a result was displayed on the Truelab® analyzer screen showing whether HLA-B27 was “DETECTED” or “NOT DETECTED”, along with the threshold cycle value(Ct value) of the internal positive control(IPC) and the validity of the test.


Out of 903 cases, there were 527 (58.3%) males and 376 (41.6%) females among which 228 (25.2%) patients belonged to the age group of 16-25 years (Fig. 1). Reactive arthritis was present in 29 patients(Fig. 2), out of which 17 (58.6%) were male and 12 (41.3%) were female. All 29 cases belonged to the age between 16 and 45 years, with the mean age of 31.5 years(Fig. 3).The axial, non-axial (peripheral) and extra-articular clinical manifestation observed in patients with ReA have been tabulated (Table 1).

Table (1):
Frequency of clinical manifestations in ReA patients29.

Pain sites No. (%)
Axial articular sites
Neck pain 13 (45%)
Lower back pain 12 (41%)
Sacroiliitis 07 (24%)
Upper back pain 06 (21%)
Peripheral articular sites
Knee pain 17 (59%)
Heel pain 15 (52%)
Dactylitis 14 (48%)
Shoulder pain 10 (34%)
Wrist pain 05 (17%)
Ankle pain 05 (17%)
Elbow joint pain 03 (10%)
Hip pain 02 (7%)
Dorsum of foot 02 (7%)
Buttocks 02 (7%)
Extra-articular sites
Uveitis 15 (52%)
Skin rashes 05 (17%)


Fig. 1. Distribution of total diarrheal samples by age and gender

Fig. 2. Frequency of Reactive arthritis in Diarrhea patients

Fig. 3. Distribution of ReA among 29 patients by age and gender

Fig. 4. Distribution of parasitic etiology among patients

Bacterial etiology of Salmonella species and parasitic etiology were identified in 20(2.2%) and 99(11%) patients out of 903 respectively (Fig. 4). The duration between diarrhea and the development of arthritic manifestations was observed to be ≥ 2 weeks. Pathogenic gut microbes identified from stool samples and their frequencies were as follows: Escherichia coli (572;63%), Klebsiella species (126; 14%), Proteus vulgaris (43; 5%), Proteus mirabilis (27; 3%), and Citrobacter species and Enterococcus species (8; 1%). The samples were further probed to determine if they induced ReA (Table 2). None of the isolated enteric pathogens were found to produce specific resistance in the antimicrobial susceptibility tests.Patients with parasitic etiology presented symptoms of spondyloarthritis post diarrhea. Four enteric pathogens (Escherichia coli, Salmonella spp., Klebsiella spp., Proteus spp., and Citrobacter spp.) were identifiedin all 29 patients presenting with symptoms of spondyloarthritides and post diarrhea (Table 2).

Table (2):
Microbiological agents in the gut associated with Reactive arthritis.

Symptoms of Post
diarrhea & gut
infection prone
to arthritis
Escherichia coli
572 (63%)
Klebsiella species
126 (14%)
99 (11%)
Proteus vulgaris
43 (5%)
Proteus mirabilis
27 (3%)
Salmonella species
20 (2%)
Enterococcus species
8 (1%)
Citrobacter species
8 (1%)

Analysis of CRP and ESR markers in 29 patients revealed that: 17(59%) patients with active inflammation had raised CRP and ESR levels, 5(17%) patients had normal levels of CRP and ESR, and 7(24%) patients had normal levels of ESR but raised CRP levels.

Out of 29 post diarrheal arthritis patients,  we identified Salmonella spp. from 13 patients, and further categorized them as HLA-B27 positive(12) and HLA-B27 negative (1).The remaining 16 patients were identified with enteric pathogens including. 13 Escherichia coli, 1 Klebsiella species, 1 Citrobacter species, and 1 Ancylostoma duodenale which triggered ReA without influencing HLA-B27.


Pathogens causing diarrhea may induce molecular mimicry between microbial products and self-antigens, resulting in the activation of auto-reactive lymphocytes, provoking immunological alterations leading to rheumatological manifestations [Schiellerup P.3, Keating R.M.7, Brocq O.10].

We have observed a higher incidence of ReA associated with HLA-B27, 12 out of 20 (41%) patients were detected with Salmonella infection, which is similar to the results in Schiellerup, et al, 2008 [Schiellerup P.3], with 6 out of 15 (40%) Salmonella infections detected with definitive ReA.

Among analysis of various Salmonella outbreak studies by Timo Hannu, 201116 & Ajene AN et. al., 201317, before and after the year of 2000, the frequency of ReA varies greatly ranging from 1.8% to 15% and 0.1% to 29% respectively. In Arthritis Rheumatology 2000, Ekman P et al. stated that following Salmonella infections, detection of HLA-B27 was associated with increased severity and duration of articular symptoms [Meadow11]. Sixteen patients had articular and extra-articular manifestations following diarrhea without influencing HLA-B27, caused by non-pathogenic isolates such as Escherichia coli, Klebsiella species, Citrobacter species and Ancylostoma duodenale. These infections may trigger ReA without development of any disease manifestations for long durations, becoming self-limited, thus not requiring treatment [Ekman P.5, Meadow11].

Ajene et al., 201317 noted, that gender does not seem to play a role in ReA associated with enteric infections. Our study, shows a male predominance of 58.6% contrary to and a study conducted by Schiellerup, et al, 20083, which reported an over-presentation of females in the ReA group. Timo Hannu, 201116, as supported in various articles, stated that although both adults and children developed ReA, adults were more susceptible to ReA following enteric infection (24%) compared to children (12%).

Twenty-nine patients with articular and extra-articular manifestations were followed up and the most commonly reported symptoms were neck pain, knee pain, heel pain and ocular symptoms such as uveitis consistent with the study conducted by Rohekar in 200813, where most patients had joint pain with another symptom such as joint stiffness or ocular symptoms or heel pain. They also observed a high rate of symptoms consistent with ReA particularly in those infected by Salmonella spp.

Garcia-Ktuzbach et al., 201812, stated that the clinical presentation mainly involves polyarthritis with dactylitis (16%), sacroiliitis (15-30%), lower back pain, enthesitis (67%) and extra articular manifestations such as uveitis, conjunctivitis, Keratoderma blenorrhagicum (KD),urethritis,and diarrhea which shows correspondence to our study which reports a similar clinical picture with sacroiliac inflammation (24%), lower back pain (41%), peripheral articular complaints such as knee pain(59%), heel pain (52%), dactylitis (48%), shoulder pain (34%) and extra-articular complications such as uveitis (52%), and Keratoderma blenorrhagicum(17%).

Arthritis symptoms following diarrhea, is a group of Spondyloarthritis (SpA), it consist of Psoriatic arthritis (PsA), Ankylosing spondylitis (AS), Reactive arthritis (ReA), Undifferentiated Spondyloarthritis (UnSpA), Childhood Reactive arthritis, and Spondylitis associated with inflammatory bowel disease (IBD).Several studies investigating the association of ReA with HLA-B27 has reported a higher frequency, but at present HLA-B27 cannot be used as a diagnostic tool for detection of ReA, although its presence is associated with a more protracted form of the arthritis18. ReA in males was more commonly associated with HLA-B27 (83%) compared to females (17%) in our study.


This study highlights the prevalence of HLA-B27 among patients with symptoms of spondyloarthritis post diarrhea. HLA typing may aid in the early diagnosis of ReA before the development of sacroiliitis and other radiological features, which are detected though MRI scan/ X-ray. In our study, we observed that 41.3% patients with infectious diarrhea were more commonly associated with HLA-B27, articular and extra-articular manifestations, suggestive of ReA triggered by bacterial etiology. The most common bacterial etiology that triggered ReA, was found to be Salmonella spp. (44.8%) and Escherichia coli (44.8%).

HLA-B27 screening can aid in early diagnosis and treatment of spondyloarthritides, in young patients and can be useful to check other family members for spondyloarthritides  as well. HLA typing can be used to exclude the other causes of inflammatory arthritis. Mandatory screening of HLA-B27 in young patients can prevent the genetic transmission to the future generation.


We would like to express our sincere gratitude to Dr. Arul Raja Murugan M.D., DM., Assistant Professor of Rheumatology, for his support in writing the manuscript and acknowledge the continuous assistance provided by our lab technicians, Mrs. Sowmyalaksmi N. and Mrs.Srividhya throughout the study period.

The authors declare that there is no conflict of interest.

All authors listed have made a substantial, direct and intellectual contribution to the work, and approved it for publication.


This study was approved from the institute’s ethical committee, SRM Medical College Hospital and Research Centre with wide Reference No: 1209/IEC/2017.

An informed consent document was obtained from all the patients.

All datasets generated or analyzed during this study are included in the manuscript.

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