Despite the availability of improved donor screening technologies and viral inactivation procedures, the risk of transmission of transfusion transmissible infections (TTIs) still remains a major concern. Constant monitoring and retrospective analysis of the incidence of TTIs, notably hepatitis B virus (HBV), hepatitis C virus (HCV), human immunodeficiency virus (HIV), and syphilis among voluntary blood donors becomes essential to evaluate the prevalence of TTIs in the population.  The current study looks at the prevalence of HIV infection amongst healthy blood donors coming to Blood Bank, J N Medical College Hospital, AMU, Aligarh during the period 2006-2010. All blood donations (voluntary or replacement) collected over this period were included. Samples were screened for anti-HIV antibody (HIV Microlisa, J Mitra & Co.) by a commercial microplate ELISA method, to determine the seropositivity of HIV infection in the donors. Of the 53422 units of blood collected over a 5- year period, 24040 (45%) were from voluntary and 29382 (55%) from replacement donors. Majority of the donors belonged to the age group of 18-40 yrs. Among the donors, 130(0.24%) were positive for anti-HIV antibody, of which 121(93.1%) were males and 09 (6.9%) were female donors. The comparative seropositivity of voluntary and replacement donors were 110(84.6%) replacement donors and 20(15.4%) voluntary donors. Motivation and recruitment of potential local blood donor population would lead to an effective voluntary system. Stringent screening of donors for transfusion transmissible infections is crucial to ensure safe supply of blood and blood products in a  well-coordinated blood transfusion service with good quality control.