To investigate into the prevalence of ETT2-harboring Escherichia coli (ETT2⁺
E. coli) infection in piglets with edema and/or diarrhea disease, a total of 90 intestinal mucosa samples were collected from 40 cases of porcine postweaning diarrhea, 32 cases of edema disease and 18 cases of diarrhea concurrent edema disease from the Jiangsu Province, China. Following first cultivating in LB broth at 37 °C for 6 h, all the samples were detected by the PCR methods, and the data show that 13 cases (32.50%) of porcine postweaning diarrhea were infected with ETT2^ÿ E. coli, while 35 cases (92.11%) and 11 cases (91.67%) were positive in edema disease and diarrhea concurrent edema disease respectively.