Coagulase-negative Staphylococci (CoNS) are common colonizers of the human skin and usually contaminants when isolated from a clinical specimen. These organisms have become increasingly recognized as agents of clinically significant nosocomial blood stream infections. The study was undertaken to identify the prevalence of clinical isolates of CoNS and their speciation. A total of 84 isolates were collected from different samples and subjected to biochemical characterization using conventional microbiological methods. 96% isolates were conveniently identified. Staphylococcus .epidermidis (36,  42.8%), S.saprophyticus (25,29.7 %), S.haemolyticus (15,17.8%),  S.lugdunensis (2,2.3 %), S.warneri (2, 2.3%),  S.cohnii (1,1.1%), and others(3,3.5%).These 3 isolates were not identified to the species level. The increasing recognition of pathogen potential CoNS and emergence of drug resistance among them, demonstrates the need to adopt simple laboratory procedure to identify CoNS and determine the prevalence and their speciation. The species identification and differentiation help in monitoring the reservoir and distribution of CoNS involved in nosocomial infections and determining the etiological agents in the hospitals.