ISSN: 0973-7510

E-ISSN: 2581-690X

Sanjita Sharma, Aarif Khan , Dinesh Kumar Dahiya, Jyoti Jain and Vishnu Sharma
Advanced Milk Testing Research Laboratory, Post Graduate Institute of Veterinary Education & Research, (Rajasthan University of Veterinary and Animal Sciences, Bikaner), Jaipur – 302020, India.
J Pure Appl Microbiol. 2015;9(1):341-348
© The Author(s). 2015
Received: 06/08/2014 | Accepted: 15/10/2014 | Published: 31/03/2015

The aim of this study was to isolate S. aureus and E. coli from raw milk samples supplied in the Jaipur city of Rajasthan along with their antibiogram patterns. Totally 160 samples were collected from different regions of Jaipur city. After aseptic collection and processing of samples, they were screened by direct plating on selective media viz. Baird-Parker Agar, EMB agar. The presumptive S. aureus and E. coli colonies were confirmed by appropriate biochemical tests. Result revealed that from screened 160 milk samples, 65 isolates (40.63 %) were of S. aureus and 57 (35.63 %) were confirmed as E. coli. Variable antibiotic susceptibility pattern (high to low) were obtained for S. aureus and E. coli isolates against the used drugs. E. coli isolates showed highest resistant towards drug Ampicillin and Penicillin G of 80.76% and 77.19%, respectively. On other side, they were moderately resistance to Nirtofurantoin (42.11%) and Oxacillin (38.60%). In case of obtained S. aureus isolates, highest resistant was noticed to Penicillin-G (86.15%), Cefotaxime (84.62%), Nirtofurantoin (81.54), Ampicillin (73.85%) followed by Chloramphenicol (69.23%) and Tetracycline (64.62%). Present findings clearly point out that the individuals consuming such contaminated raw milk and its products from this region are at high risk of getting ill and might to develop antibiotic resistance.


S. aureus, E. coli, Milk, Antibiotic Resistance

Article Metrics

Article View: 668

Share This Article

© The Author(s) 2015. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.