A total of five different Lactic acid bacterial species were identified from yoghurt samples collected from Chittagong region of Bangladesh. Among the isolates, the prevalence of Lactobacillus delbrueckii and Lactobacillus plantarum were 111 and 114cfu/ml respectively. After identification and isolation, we have optimized the suitable growth of isolates against pH, temperature and various concentration of NaCl. The results showed that better growth of organisms was observed in the presence of 3-6% NaCl. These species can tolerate up to 3% bile salt but the best tolerance found at 1% bile salt. To evaluate the antimicrobial properties of identified species, growth inhibition test has been done against some selected pathogens. It showed that Lactobacilli inhibited the growth of all pathogenic bacterial species. Inhibition by isolates and further treated with papain indicate that our isolates inhibited the growth of pathogenic microorganisms by producing Bacteriocins like substances.The selected species were subjected to Antibiotic Susceptibility testing to observe their features of carrying Antibiotic resistance genes.  Among the 21 Antibiotics 17 antibiotics found resistant to Lactobacillus delbrueckii, on the other hand, Lactobacillus plantarum were found resistant to 4 antibiotics.