Enterococcus is an important pathogen all around the world, known as an important nosocomial pathogen. This study determined the strains and antibiotics susceptibility of Enterococci isolated from clinical samples in Imam Ali Hospital in Kermanshah, Iran, from July 2014 to July 2015. This experimental study was performed on 58 Enterococci strains isolated from clinical samples in Imam Ali Hospital in Kermanshah, Iran. Patients’ information was collected by a questionnaire. After isolating and identifying 58 strains of Enterococci from clinical samples, complimentary tests were carried out. Antibiogram test was performed by Kirby-Bauer disk diffusion method and CLSI criteria. Statistical analysis was performed using the SPSS 16. The frequencies of the isolated Enterococci species included E. faecalis with 72.41% (42 cases), E. feacium with 13.79% (8 cases), E. hirea with 6.9% (4 cases), E. avium with 3.44% (2 cases), E. gallinarium with 1.73% (1 case), and E. mundtii with 1.73% (1 case). Most of Enterococci strains were isolated from urine samples with 48.27% (28 cases). The highest and lowest resistances were to penicillin (95%) and linezolid (0%), respectively. We found a significant statistical difference between Enterococcus and time of hospital stay (P = 0.03). Based on the results of the present study, E. faecalis was, by far, the most predominant isolate. This suggests that in order to prevent the antibiotic resistance and select an appropriate antibiotic before the treatment, antibiogram test should be done for each patient.