Entomopathogenic fungi are among the agents of biological control most widely studied for their ubiquity, easy and fast growth on different substrates and its wide host range. In this study 30 entomopathogenic fungi isolated from soils where citrus is grown in different regions of Mexico to assess the production of chitinases, lipases and proteases on solid media by calculating the enzymatic index, a criterion that relates the growth rate and enzyme activity.We analyzed by ANOVA with mean separation by a Tukey test (±= 0.05). The results do not meet the criteria of normality were analyzed using a Kruskal-Wallis test. According to the results of this test for protease and lipase activity at least one of the isolated is different regarding their enzymatic index. Respect to chitinase activity the according to the ANOVA there is significative difference in chitinolytic activity (F = 2.44; p = 0.001) between the strains tested; It was observed that the chitinase activity was exhibited in a range 1.10-1.50; the highest enzymatic index value was for the isolate HIB-19, followed by the isolate HIB-1. These results show the presence of various hydrolytic enzymes from fungi evaluated.