In  contrast to the Lactobacillus-dominated healthy genital organ, bacterial infection of female genital organwas associated with a range of negative outcome acquisitions including genital transmitted diseases, preterm births, and pelvic inflammatory. So long, laboratory diagnosis of bacterial infection of women reproductive organ depended on Gram-stained swabs and microscopic observation. In this study, We  described an easy  molecular method for the identification of  Lactobacilli species  by  amplified   primers : (LactoF,   LactoR,    LcrisF, LcrisR,   LjensF,   LjensR,   LgassF, LgassR,   LinersFLinersR )  of Lactobacilli  specie specific.  Sixty swab samples from healthy women and sixty from infected; ranging their ages from 20- 40 years were donated freshly by Alhabib Hosbital and Malaz Clinic, Riyadh Saudi Arabia during 2012 respectively. Microbial DNA was extracted by two methods, extraction kit and boiling. PCR mixtures were prepared. This study was aimed to identify Lactobacillus species from healthy and unhealthy women.Our results did not provide support for the findings of YAN Dong-hui et al in Chinese women and Elahe Motevaseli et al of Iranian healthy and unhealthy women that L. crispatus, L. gasseriand L. jenseniiwere only dominant spices in healthy women. In contrast to their findings, we found that L. crispatus, L. gasseri, L. iners were dominant in Saudi healthy women although their dominant numbers were significantly different, while, L. jensenii was dominant only in unhealthy women.