ISSN: 0973-7510

E-ISSN: 2581-690X

Keshavarz Rouhollah1, Mosavari Nader1 and Maham Masood2
1Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization(AREEO), Tehran , Iran.
2Institute of Biotechnology, Urmia University, Urmia, Iran.
J. Pure Appl. Microbiol., 2016, 10 (1): 395-399
© The Author(s). 2016
Received: 11/02/2016 | Accepted: 19/04/2016 | Published: 31/03/2016
Abstract

Bovine tuberculosis is a zoonotic disease that is caused by Mycobacterium bovis. Rapid detection of disease can play an important role for controlling; Therefore researches for developing of rapid diagnostic tests with high sensitivity and specificity are considered. Patho TB test is one of considering tests for rapid detection in human that has been developed recently. In this study using of patho TB test for suspected lesion of bovine tuberculosis in cattle were examined. One hundred suspected samples that were transferred to bovine tuberculosis reference laboratory in Iran were evaluated by classic culture as gold standard, MGIT culture, PCR and patho Tb test; sensitivity and specificity of each one were determined. By performed investigations, sensitivity and specificity of MGIT culture were 100%. Also sensitivity and specificity of PCR and patho Tb test were evaluated 100% and 88% respectively. The results have been shown patho-TB test can be used for detection of bovine tuberculosis in suspected lesions with high viscosity either.

Keywords

Patho-TB Test, MGIT, PCR, Mycobacterium bovis.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.