Streptomyces antibiotic regulatory proteins (SARP) normally play positive regulatory roles during streptomyces antibiotic biosynthesis. sare_4854, a gene in Salinispora arenicola CNH643 DSM 44819 was proposed to encode a SARP. Here, sare_4854 was expressed in a heterologous system for functional characterization. However, the antibiotic production in the resultant strain was repressed significantly. Bioinfomatic analysis showed that Sare_4854 has an additional nucleoside triphosphate hydrolases (NTPase) domain at the C-terminus, besides a SARP-like domain (DBD: DNA binding domain and BTAD: bacterial transcriptional activation domain) at the N-terminus conserved in all SARPs. The possible mechanism of Sare_4854 in antibiotic synthesis in this abnormal phenomenon was discussed here.
SARP, Sare_4854, antibiotic synthesis, Streptomyces
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