ISSN: 0973-7510

E-ISSN: 2581-690X

Xiao Shu1#, Wei Zhang1#, Jinlong Yu1#, Shaowen Wu1#, Yanling Ma1, Minzhe Zhu1, Sisi Xia1, Shichao Xia1, Hao Zhang1, Aiying Li1,2 and Chao Qi1*
1Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Huazhong Normal University, Wuhan 430079, China.
2School of Life Sciences, State Key Laboratory of Microbial Technology, Shandong University, Jinan – 250 100, China.
J Pure Appl Microbiol. 2014;8(2):1447-1452
© The Author(s). 2014
Received: 09/01/2014 | Accepted: 18/03/2014 | Published: 31/04/2014
Abstract

Streptomyces antibiotic regulatory proteins (SARP) normally play positive regulatory roles during streptomyces antibiotic biosynthesis. sare_4854, a gene in Salinispora arenicola CNH643 DSM 44819 was proposed to encode a SARP. Here, sare_4854 was expressed in a heterologous system for functional characterization. However, the antibiotic production in the resultant strain was repressed significantly. Bioinfomatic analysis showed that Sare_4854 has an additional nucleoside triphosphate hydrolases (NTPase) domain at the C-terminus, besides a SARP-like domain (DBD: DNA binding domain and BTAD: bacterial transcriptional activation domain) at the N-terminus conserved in all SARPs. The possible mechanism of Sare_4854 in antibiotic synthesis in this abnormal phenomenon was discussed here.

Keywords

SARP, Sare_4854, antibiotic synthesis, Streptomyces

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