Possible Negative Regulatory Effects of Antibiotic Production in a Hetrologous System by Sare_4854, a Novel Member of Streptomyces Antibiotic Regulatory Protein Family (SARP) from a Marine Microorganism Salinispora arenicola CNH643 DSM 44819

ISSN: 0973-7510

E-ISSN: 2581-690X

Possible Negative Regulatory Effects of Antibiotic Production in a Hetrologous System by Sare_4854, a Novel Member of Streptomyces Antibiotic Regulatory Protein Family (SARP) from a Marine Microorganism Salinispora arenicola CNH643 DSM 44819

Xiao Shu¹#, Wei Zhang¹#, Jinlong Yu¹#, Shaowen Wu¹#, Yanling Ma¹, Minzhe Zhu¹, Sisi Xia¹, Shichao Xia¹, Hao Zhang¹, Aiying Li^1,2 and Chao Qi¹*

¹Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Huazhong Normal University, Wuhan 430079, China.

²School of Life Sciences, State Key Laboratory of Microbial Technology, Shandong University, Jinan – 250 100, China.

J Pure Appl Microbiol. 2014;8(2):1447-1452

Received: 09/01/2014 | Accepted: 18/03/2014 | Published: 31/04/2014

Abstract

Streptomyces antibiotic regulatory proteins (SARP) normally play positive regulatory roles during streptomyces antibiotic biosynthesis. sare_4854, a gene in Salinispora arenicola CNH643 DSM 44819 was proposed to encode a SARP. Here, sare_4854 was expressed in a heterologous system for functional characterization. However, the antibiotic production in the resultant strain was repressed significantly. Bioinfomatic analysis showed that Sare_4854 has an additional nucleoside triphosphate hydrolases (NTPase) domain at the C-terminus, besides a SARP-like domain (DBD: DNA binding domain and BTAD: bacterial transcriptional activation domain) at the N-terminus conserved in all SARPs. The possible mechanism of Sare_4854 in antibiotic synthesis in this abnormal phenomenon was discussed here.

Keywords

SARP, Sare_4854, antibiotic synthesis, Streptomyces

Download PDF

Article Metrics

Article View: 1053

JPAM.8.2.75 (301 downloads)

Share This Article

© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.