ISSN: 0973-7510

E-ISSN: 2581-690X

P. Ezilrani1, J. Godwin Christopher1 and K. Karal Marx2
1Molecular and Microbiology Research Laboratory, Environmental Biotechnology division, School of BioSciences and Technology, VIT University, Vellore – 632 014, India.
2Fisheries biotechnology Centre, Department of Aquaculture, Fisheries College and Research Institute, Thoothukudi – 628 008, India.
J Pure Appl Microbiol. 2015;9(Spl. Edn. 1):623-632
© The Author(s). 2015
Received: 08/01/2015 | Accepted: 24/03/2015 | Published: 31/05/2015
Abstract

Genetic variation between six populations of Clarias gariepinus were analyzed using six microsatellite loci. All the loci were highly polymorphic among all samples. The mean number of alleles and effective alleles per locus ranged from 4.667 to 5.833 and 2.000 to 6.000 respectively. The heterozyosities observed were found to be 0.739±0.012. Conformity to Hardy Weinberg Equilibrium using chi-square test showed that at 26 test out of 36 test (P<0.005) showed a significant value. The population differentiation (FST= -0.0170) was lower than gene flow (Nm= 0.8885) indicating heterozygosities in the population. The polymorphism information content (PIC) were greater than 0.6 in most of the loci indicating informative marker for the selected loci. The Nei’s genetic distance was high between Pop1 and Pop6 with 5.669. The observations showed that due to allelic diversity and estimates of heterozygosity, these markers can be useful in the genus Clarias for population level analysis in the Indian Clariidae fishes using microsatellite DNA markers.

Keywords

Genetic variability, Polymorphism, Genetic distance, Neutrality, AMOVA

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