ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Raed Obaid Saleh1 , Rana Hussein Raheema2 and Zahraa J. Jameel3
1Department of Medical  Laboratory Techniques , Al-maarif University College, Al-Anbar Iraq.
2Department of Microbiology ,Medical College , Wasit University Al-kut, Iraq.
3Department of Biology, College of Science  Diyala University, Diyala, Iraq.
J. Pure Appl. Microbiol., 2018, 12 (4): 2199-2204 | Article Number: 5366
Received: 30/10/2018| Accepted: 12/12/2018 |Published: 30/12/2018
Abstract

In this study, sixty specimens were collected of Staphylococcus aureus isolation from several sources but found twenty-five specimens of Staphylococcus aureus isolated from skin from External laboratories in Baghdad, Iraq, during the period of December to May 2018. Then the samples were inoculated on culture media (Mannitol agar). The results of the sequencing showed congruence with isolation Staphylococcus aureus of amplified product of 16S rRNA gene appeared 99%compatibility. The results as shown After alignment of product amplification of 16S rRNA having seven Transition(A>G, A>G, T>C, T>C, G>A, C>T and A>G, and having three Transversion (T>G, G>C and A>T) from the Gene Bank.Registration of Iraqi isolate for Staphylococcus aureus bacteria in National Center for Biotechnology Information and under the accession number MH 145371.1. and it is available for download at NCBI: https://www.ncbi.nlm.nih.gov/nuccore/ MH 145371.1. The aim of the study was to determine the phylogenetic tree of Staphylococcus aureus based on 16S rRNA sequencing.The local Iraq isolate was registered after the correspondence of (NCBI), and obtained accession number and became a reference to Iraq and the Middle East and the world found variations of the local strain on the world.

Keywords

Staphylococcus aureus, 16SrRNA  gene, Skin infections.

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