Phylogenetic Relationships of Escherichia coli Isolates Associated with Bovine Fecal and Milk samples

ISSN: 0973-7510

E-ISSN: 2581-690X

Phylogenetic Relationships of Escherichia coli Isolates Associated with Bovine Fecal and Milk samples

Ashgan M. Hessain¹ , Abdullah A. Al-Arfaj², Moussa I. Mohamed³ and Mohamed I. AlHazmi⁴

¹Associate Prof. of Microbiology, Department of Health Science, College of Applied Studies and Community Service, King Saud University, P. O. Box 22459 Riyadh11495, Saudi Arabia.

²Assistant Prof. of Microbiology, Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.

³Prof. of Microbiology, Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.

⁴Department of Food and Nutrition Sciences, College of Food and Agriculture Science, King Saud University, Saudi Arabia.

J Pure Appl Microbiol. 2013;7(Spl. Edn.: November):01-12

Received: 11/08/2013 | Accepted: 22/10/2013 | Published: 30/11/2013

Abstract

To study the genetic diversity among Escherichia coli strains isolated from bovine fecal and milk samples, nineteen isolates of E. coli were typed by random amplification of polymorphic DNA (RAPD). Clusters of strains were defined on the basis of the epidemiological data and subsequently phylogenetic tree was created and revealed 4 clusters. The obtained results indicated that the serotypes causing bovine mastitis were similar genetically to that causing diarrhea. This type of information can be used in clinical setting to discriminate ongoing epidemics of E. coli from incidentally increased infection rate.

Keywords

Phylogenetic tree, Escherichia coli, RAPD

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