Anaerobic bacterium (strain SN9) with simultaneous capabilities of denitrification and sulfate reduction was enriched and isolated in a specially designed medium by anaerobic Hungate technology. The characterization of SN9 was conducted from the morphological, physiological and phylogenetic aspects. It was a rod-shaped, gram-negative, 0.2-0.8 µm in width and 2.0-5.0 µm in length. Most of the fatty acids of SN9 distributed among C12:0~C19-CYC-FAME and the main fatty acids consisted of C14:0 FAME, C16:0-FAME, C18:1c-FAME,C18:0-FAME,C19-CYC-FAME. Based on the morphological and physiological characteristics as well as the phylogenetic analysis, SN9 was identified as Bacillus coagulans since its 16S rDNA beared 99% resemblance to that of Paenibacillus lautus SN9 (Accession No. DQ450463). The removal efficiencies of NO3– and SO42- were up to 80.78% and 96.22 % when the initial concentrations of NO3– and SO42- were 3812.59 mg/L and 552.52 mg/L, respectively. The characterization of the present isolate had important ecological implications in treating organic wastewater containing high concentration of sulfate and nitrogen.
Strain with simultaneous denitrifying and sulfate reducing capabilities, Characterization, Identification of fatty acids, Function verification
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