Microbiological quality of an indoor air is one of the indicators of proper hygienic conditions in a health care hospital. This study intends to assess the bacteriological profile of an indoor air by settle plate method. About 100 air samples were collected from various locations in the hospital by using 5% Sheep blood agar and Sabouraud dextrose agarculture plates. Frequent isolation of common aerobic bacterial flora like Staphylococcus aureus (27.72%) followed by Diptheroids (24.75%), Bacillus spp. (24.09%), Micrococcus (23.43%) were observed. Among fungal isolates Aspergillus niger (25.00%) were more prevalent followed by Zygomyces (23.07%), Fusarium spp. (21.15%), Aspergillus flavus (17.30%) and Aspergillus fumigatus (13.46%) in the hospital environment. Most of the isolates identified were a part of the normal aerobic microbial flora; however, a minimum degree of bacterial and fungal load were observed in the casualty and in the wards due to the constant patient traffic and unrestricted access to patients.