This paper presents an extraction and antimicrobial study of essential oil obtained from Nigella sativa seeds. Extraction using polar solvent resulted in higher recovery of desired bioactive compounds, thymoquinone and thymol. It was found that extraction of antioxidant (i.e. Agidol 7) favours aqueous solvent, i.e. 50% ethanol and 50% methanol. The highest TPC, TFC and antioxidant activities were obtained at power 224W and temperature of 50 °C. This study shows that N. sativa seed oil has an effective antibacterial activity from the in-vitro studies. The result showed that the essential oil extracted using n-hexane has the highest (30.17%) effectiveness against E. coli.
Nigella sativa, Ultrasonic assisted extraction, Flavonoid, Thymol, Thymoquinone, Antimicrobial
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