An extracellular protease produced by Arthrobacter globiformis, an isolate from the gut of kola nut weevil Balanogastris kolae Desbr was partially purified and characterized in this study. The enzyme showed optimum activity at temperature of 60^oC and pH 8.0. The molecular weight was estimated to be 35.7 kDa, while the K_m and V_max for the hydrolysis of casein were 71.43 mg/min/mL and 8.33mg/ mL respectively. The enzyme retained almost 80% of its relative activity after 20 minutes incubation at 60^oC and its activity was not particularly influenced by majority of the cations tested. However, it was inhibited by Hg²⁺ and Fe³⁺.