ISSN: 0973-7510

E-ISSN: 2581-690X

Ranjna Sharma1 , Sheetal Rana1, S.P. Singh2 and Mohinder Kaur3

1PhD (Microbiology) Scholar.
2Scientist C (National Agri-Food Biotechnology Institute, Mohali, Punjab, India.
3Department of Basic Sciences, College of Forestry, Dr. YS Parmar University of Horticulture & Forestry, Nauni, Solan-173230 Himachal Pradesh, India.
J. Pure Appl. Microbiol. 2014, 8(6):4715-4718
© The Author(s). 2014
Received: 20/07/2014 | Accepted: 09/09/2014 | Published: 31/12/2014
Abstract

An efficient phosphate solubilizing Pseudomonas aeruginosa strain An-G isolated from apple rhizosphere of Himachal Pradesh, exhibited solubilization of tricalcium phosphate (TCP) in Pikovskaya’s broth (47µg/ml). A decline in the pH of the medium was observed after three days of incubation at 28±2oC from 7.0 – 4.02 during the solubilization of phosphate substrate (TCP). Among the different organic acids which were estimated through HPLC-MS/MS, succinic acid was produced in higher concentration (2.016µg/ml). Other major organic acids produced were citric acid, malic acid and malonic acid in concentration 0.162 µg/ml, 0.20 µg/ml and 0.39µg/ml respectively. The production of fumaric acid, tartaric acid, quinic acid, lactic acid and schimic acid were observed in small amount during the solubilization of tricalcium phosphate.

Keywords

Pseudomonas aeruginosa, Phosphate solubilizing bacteria, TCP and Organic acids

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