An efficient phosphate solubilizing Pseudomonas aeruginosa strain An-G isolated from apple rhizosphere of Himachal Pradesh, exhibited solubilization of tricalcium phosphate (TCP) in Pikovskaya’s broth (47µg/ml). A decline in the pH of the medium was observed after three days of incubation at 28±2oC from 7.0 – 4.02 during the solubilization of phosphate substrate (TCP). Among the different organic acids which were estimated through HPLC-MS/MS, succinic acid was produced in higher concentration (2.016µg/ml). Other major organic acids produced were citric acid, malic acid and malonic acid in concentration 0.162 µg/ml, 0.20 µg/ml and 0.39µg/ml respectively. The production of fumaric acid, tartaric acid, quinic acid, lactic acid and schimic acid were observed in small amount during the solubilization of tricalcium phosphate.
Pseudomonas aeruginosa, Phosphate solubilizing bacteria, TCP and Organic acids
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