A study was conducted to find out the potential of castor seed coat waste for production of cellulase by Aspergillus niger. The powdered waste was fermented in a flask containing mineral salt medium. Various process parameters were optimized for enhancing the yield of cellulase. The maximum activity of CMCase (28.14 U/ gram dry substrate) and FPase (18.14 U/ gram dry substrate) were recorded under optimum conditions of inoculum size (9 discs), incubation period (120h), substrate concentration (7g), pH (5.5), temperature (35°C), moisture level (1:3), CMC (0.5%) as carbon source and pepton (0.5%) as nitrogen source. From this study, it can be concluded that Aspergillus niger holds the potential of converting castor seed coat waste into cellulose.
CMCase and FPase activity, agricultural waste, optimization
Share This Article
© The Author(s) 2015. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.