ISSN: 0973-7510

E-ISSN: 2581-690X

Subhash Ghodadara , Prateek Shilpkar and Arvind Dungrechiya
1Department of Microbiology and Biogas Research and Extension Centre, Gujarat Vidyapith, Sadara, District- Gandhinagar Gujarat, India.
J Pure Appl Microbiol. 2015;9(1):623-629
© The Author(s). 2015
Received: 23/10/2014 | Accepted: 05/12/2014 | Published: 31/03/2015
Abstract

A study was conducted to find out the potential of castor seed coat waste for production of cellulase by Aspergillus niger. The powdered waste was fermented in a flask containing mineral salt medium. Various process parameters were optimized for enhancing the yield of cellulase. The maximum activity of CMCase (28.14 U/ gram dry substrate) and FPase (18.14 U/ gram dry substrate) were recorded under optimum conditions of inoculum size (9 discs), incubation period (120h), substrate concentration (7g), pH (5.5), temperature (35°C), moisture level (1:3), CMC (0.5%) as carbon source and pepton (0.5%) as nitrogen source. From this study, it can be concluded that Aspergillus niger holds the potential of converting castor seed coat waste into cellulose.

Keywords

CMCase and FPase activity, agricultural waste, optimization

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