In a laboratory study 18 different types of bacteria were isolated from soil showing protease production in 1% casein agar medium. After screening, one potent bacterial culture was identified as Bacillus thuringiensis and its protease production was further optimized for various fermentation conditions. Results show that the culture gives maximum alkaline protease production under optimum condition of pH- 11.5 (40.7 U/mL), temperature- 25ºC (60.13 U/mL), substrate concentration- 3% (33.54 U/mL) inoculum size – 1mL (v/v) with optimum activity 60.13 U/mL, incubation time- 24 hr (60.13 U/mL), 0.30% beef extract as nitrogen source (61.23 U/mL), and 1% lactose as carbon source (37.77 U/mL).
Bacillus thuringiensis, alkaline protease, pods of Cassia fistula
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