ISSN: 0973-7510

E-ISSN: 2581-690X

Misha V. Patel , Prateek Shilpkar and Arvind Dungrechia
1Biogas Research Centre and Post Graduate Department of Microbiology, Gujarat Vidyapeeth, Sadra 382 320, Gandhinagar, Gujarat, India.
J Pure Appl Microbiol. 2015;9(1):791-795
© The Author(s). 2015
Received: 15/11/2014 | Accepted: 21/12/2014 | Published: 31/03/2015

In a laboratory study 18 different types of bacteria were isolated from soil showing protease production in 1% casein agar medium. After screening, one potent bacterial culture was identified as Bacillus thuringiensis and its protease production was further optimized for various fermentation conditions. Results show that the culture gives maximum alkaline protease production under optimum condition of pH- 11.5 (40.7 U/mL), temperature- 25ºC (60.13 U/mL), substrate concentration- 3% (33.54 U/mL) inoculum size – 1mL (v/v) with optimum activity 60.13 U/mL, incubation time- 24 hr (60.13 U/mL), 0.30% beef extract as nitrogen source (61.23 U/mL), and 1% lactose as carbon source (37.77 U/mL).


Bacillus thuringiensis, alkaline protease, pods of Cassia fistula

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