ISSN: 0973-7510

E-ISSN: 2581-690X

Misha V. Patel , Prateek Shilpkar and Arvind Dungrechia
1Biogas Research Centre and Post Graduate Department of Microbiology, Gujarat Vidyapeeth, Sadra 382 320, Gandhinagar, Gujarat, India.
J Pure Appl Microbiol. 2015;9(1):791-795
© The Author(s). 2015
Received: 15/11/2014 | Accepted: 21/12/2014 | Published: 31/03/2015
Abstract

In a laboratory study 18 different types of bacteria were isolated from soil showing protease production in 1% casein agar medium. After screening, one potent bacterial culture was identified as Bacillus thuringiensis and its protease production was further optimized for various fermentation conditions. Results show that the culture gives maximum alkaline protease production under optimum condition of pH- 11.5 (40.7 U/mL), temperature- 25ºC (60.13 U/mL), substrate concentration- 3% (33.54 U/mL) inoculum size – 1mL (v/v) with optimum activity 60.13 U/mL, incubation time- 24 hr (60.13 U/mL), 0.30% beef extract as nitrogen source (61.23 U/mL), and 1% lactose as carbon source (37.77 U/mL).

Keywords

Bacillus thuringiensis, alkaline protease, pods of Cassia fistula

Article Metrics

Article View: 1138

Share This Article

© The Author(s) 2015. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.