Production of esterase enzyme which is the key enzyme for organophosphorus pesticide degradation from Bacillus subtilis KPA-1 was confirmed in present study. Esterase production from Bacillus subtilis KPA-1 was observed when inoculated in mineral salt medium and localization of esterase production was also determined and the esterase enzyme was found to be membrane bound enzyme. Various process parameters were optimized by conventional method for maximum esterase production by B. subtilis KPA-1. Results indicate that pH 8, incubation temperature of 40°C, incubation time of 72 hr, 48 hr old inoculum with 3% inoculum size, glucose as carbon source and ammonium sulfate as nitrogen source and 800ppm monocrotophos concentration were found to be optimum values for highest esterase production.
Pesticide degradation, Fermentation conditions, Esterase.
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