ISSN: 0973-7510

E-ISSN: 2581-690X

Komal P. Acharya , Prateek Shilpkar and Mayur C. Shah
Department of Microbiology and Biogas Research and Extension Centre, Gujarat Vidyapeeth, At & Post- Sadara, District- Gandhinagar, Gujarat- 382 320, India.
J. Pure Appl. Microbiol., 2016, 10 (3): 2079-2087
© The Author(s). 2016
Received: 06/04/2016 | Accepted: 25/05/2016 | Published: 30/09/2016
Abstract

Production of esterase enzyme which is the key enzyme for organophosphorus pesticide degradation from Bacillus subtilis KPA-1 was confirmed in present study. Esterase production from Bacillus subtilis KPA-1 was observed when inoculated in mineral salt medium and localization of esterase production was also determined and the esterase enzyme was found to be membrane bound enzyme. Various process parameters were optimized by conventional method for maximum esterase production by B. subtilis KPA-1. Results indicate that pH 8, incubation temperature of 40°C, incubation time of 72 hr, 48 hr old inoculum with 3% inoculum size, glucose as carbon source and ammonium sulfate as nitrogen source and 800ppm monocrotophos concentration were found to be optimum values for highest esterase production.

Keywords

Pesticide degradation, Fermentation conditions, Esterase.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.