ISSN: 0973-7510

E-ISSN: 2581-690X

Maohua Qu, Juan Du and Weiping Chen
Key Lab of Agricultural Products processing and quality control of Nanchang City, Jiangxi Agricultural University, Nanchang – 330045, China.
J Pure Appl Microbiol. 2014;8(Spl. Edn. 2):231-238
© The Author(s). 2014
Received: 12/06/2014 | Accepted: 14/08/2014 | Published: 30/11/2014
Abstract

The medium was optimized for trehalose synthase production by Corynebacterium Glutamicum. Effects of factors on trehalose synthase production were examined to improve trehalose synthase yield. Experimental designs were applied to optimize medium. Results of factional factorial design (FFD) showed that maltose, NaCl, K2HPO4 and beef extract were the most significant factors affecting trehalose synthase production. Further central composite design (CCD) experiments showed that optimal maltose and NaCl concentrations were 22g/L and 13.36g/L, respectively. Trehalose synthase activity increased to 580 U/mL, an approximate 1.76-fold increase over the previous activity (330 U/mL) using an optimized fermentation medium with the main composition maltose (22g/L), beef extract (7.49g/L), NaCl (13.36g/L), K2HPO4(11.33g/L) and peptone
(15.00g/L).

Keywords

Trehalose synthase, Optimization, Response surface methodology

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